中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (34): 5467-5473.doi: 10.3969/j.issn.2095-4344.2017.34.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

细菌纳米纤维素/壳聚糖复合管制备及其作为小径人工血管潜力的评价

李  雪,唐敬玉,包露涵,陈  琳,洪  枫
  

  1. 东华大学化学化工与生物工程学院,上海市  201620
  • 收稿日期:2017-07-24 出版日期:2017-12-08 发布日期:2018-01-04
  • 通讯作者: 洪枫,教授,博士生导师,东华大学化学化工与生物工程学院,上海市 201620
  • 作者简介:李雪,女,1990年生,安徽省蚌埠市人,2017年东华大学毕业,硕士,主要从事生物技术与生物医用材料研究。
  • 基金资助:

    国家自然科学基金(51373031);教育部新世纪优秀人才支持计划(NCET-12-0828);上海市科委“科技创新行动计划”项目(15520720800);中央高校基本科研业务费专项资金资助项目

Preparation and evaluation of bacterial nano-cellulose/chitosan composite tubes as potential small-diameter vascular grafts

Li Xue, Tang Jing-yu, Bao Lu-han, Chen Lin, Hong Feng
  

  1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
  • Received:2017-07-24 Online:2017-12-08 Published:2018-01-04
  • Contact: Hong Feng, Professor, Doctoral supervisor, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
  • About author:Li Xue, Master, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
  • Supported by:
    the National Natural Science Foundation of China, No. 51373031; the Program for New Century Excellent Talents in University, No. NCET-12-0828; the Science and Technology Commission of Shanghai Municipality, No. 15520720800; and the Fundamental Research Funds for the Central Universities

摘要:

文章快速阅读:

 

文题释义:
细菌纳米纤维素/壳聚糖复合管:该纳米纤维素管由醋酸杆菌在一种自主设计的双硅胶管反应器中原位培养制备而来,已获国家发明专利保护。其特征在于管壁的纳米纤维网络的持水量达到185 g/g,网络结构均匀且不分层,管壁的内、外表面具有相似的纤维网络形貌,机械力学性能优良,其湿态轴向拉伸强度和爆破强度可分别达到200 kPa和105 kPa,可用作制备复合材料的基底材料及管状组织工程支架等。通过在纤维表面沉积不同量的壳聚糖制备细菌纳米纤维素/壳聚糖复合管,很好地保留了纳米纤维网络形貌。该复合管具有良好的生物相容性,是潜在的小径血管替代品。
小径人工血管:小径人工血管一般指内径< 6 mm的人造血管,应用于小口径病变血管的替换。然而,由于小径血管内血流量小,易堵塞等原因,临床上尚没有成功应用的产品,因此众多研究者将目光投向了小径人工血管的研发。当前的研究热点主要集中在更优生物相容性材料的应用和组织工程血管的开发。实验以细菌纳米纤维素和壳聚糖2种天然材料制备复合小口径管,具有优良的性能,是一种潜在的小径血管替代品和组织工程支架。
 
背景:目前临床上仍旧缺乏人工血管用于小口径血管(< 6 mm)的替换。
目的:探究细菌纳米纤维素/壳聚糖复合管作为小口径人工血管的可行性。
方法:将培养制备的细菌纳米纤维素管分别与0.5%,1%,2%的壳聚糖复合,制备细菌纳米纤维素/壳聚糖复合管。对这些管材进行宏观形态、微观结构、密度、持水量、爆破压、机械力学性能以及血液相容性和细胞相容性等表征。
结果与结论:①与细菌纳米纤维素管相比,细菌纳米纤维素/壳聚糖复合管的水渗透性减小,密度和持水能力均增大,轴向和径向力学性能增强,但是延展性和弹性降低;②对于血液相容性而言,细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管的溶血率均能满足医用材料要求,但是血浆复钙时间测试结果显示,细菌纳米纤维素/壳聚糖管的抗凝性略弱于细菌纳米纤维素管;③细胞相容性实验表明,猪髋动脉内皮细胞在细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管上均能稳定增殖生长,但是在细菌纳米纤维素/壳聚糖管上的增殖效果没有细菌纳米纤维素管明显。

关键词: 生物材料, 纳米材料, 细菌纳米纤维素, 壳聚糖, 复合管状材料, 血液相容性, 细胞相容性, 小口径人工血管, 国家自然科学基金

Abstract:

BACKGROUND: There are still no applicable vascular grafts for clinical replacement of the small-diameter blood vessels (< 6 mm).
OBJECTIVE: To explore the possibility of bacterial nano-cellulose (BNC)/chitosan (CH) composite tubes as small-diameter artificial blood vessels.
METHODS: BNC/CH tubes were fabricated by introducing chitosan (0.5%, 1%, 2%) into BNC tubes. Physicochemical properties of BNC/CH tubes were analyzed including macro-morphology, microstructure, density, water-holding capacity, burst pressure, mechanical properties, blood compatibility and cytocompatibility.
RESULTS AND CONCLUSION: The results indicated that, as compared with the BNC tubes, the water permeability of BNC/CH tubes reduced, the density and water-holding capacity increased, as well as the axial and radial mechanical performance was enhanced, but ductility and elasticity of BNC/CH tubes were weakened. Regarding blood compatibility, hemolysis ratios of BNC and BNC/CH met the requirements of medical grade composites, while the plasma recalcification time showed that anticoagulation of BNC/CH tubes was slightly weaker than that of the BNC tubes. Cell compatibility experiments showed that porcine hip artery endothelial cells stably proliferated on the inner surface of both BNC and BNC/CH tubes, but the proliferation was more obvious on the BNC surface than on the BNC/CH surface

Key words: Cellulose, Chitosan, Blood Vessel Prosthesis, Tissue Engineering

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